N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans.
Identifieur interne : 000449 ( Main/Exploration ); précédent : 000448; suivant : 000450N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans.
Auteurs : Edouard Evangelisti [Royaume-Uni] ; Temur Yunusov [Royaume-Uni] ; Liron Shenhav [Royaume-Uni] ; Sebastian Schornack [Royaume-Uni]Source :
- BMC microbiology [ 1471-2180 ] ; 2019.
Descripteurs français
- KwdFr :
- Arylamine N-acetyltransferase (génétique), Colorants fluorescents (MeSH), Gentamicine (pharmacologie), Isoenzymes (génétique), Maladies des plantes (MeSH), Phytophthora (effets des médicaments et des substances chimiques), Phytophthora (enzymologie), Phytophthora (génétique), Phytophthora infestans (effets des médicaments et des substances chimiques), Phytophthora infestans (enzymologie), Phytophthora infestans (génétique), Résistance aux substances (génétique), Transformation génétique (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Phytophthora, Phytophthora infestans.
- enzymologie : Phytophthora, Phytophthora infestans.
- génétique : Arylamine N-acetyltransferase, Isoenzymes, Phytophthora, Phytophthora infestans, Résistance aux substances.
- pharmacologie : Gentamicine.
- Colorants fluorescents, Maladies des plantes, Transformation génétique.
English descriptors
- KwdEn :
- Arylamine N-Acetyltransferase (genetics), Drug Resistance (genetics), Fluorescent Dyes (MeSH), Gentamicins (pharmacology), Isoenzymes (genetics), Phytophthora (drug effects), Phytophthora (enzymology), Phytophthora (genetics), Phytophthora infestans (drug effects), Phytophthora infestans (enzymology), Phytophthora infestans (genetics), Plant Diseases (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Arylamine N-Acetyltransferase, Isoenzymes.
- drug effects : Phytophthora, Phytophthora infestans.
- enzymology : Phytophthora, Phytophthora infestans.
- genetics : Drug Resistance, Phytophthora, Phytophthora infestans.
- chemical , pharmacology : Gentamicins.
- chemical : Fluorescent Dyes, Plant Diseases, Transformation, Genetic.
Abstract
BACKGROUND
Oomycetes are pathogens of mammals, fish, insects and plants, and the potato late blight agent Phytophthora infestans and the oil palm and cocoa infecting pathogen Phytophthora palmivora cause economically impacting diseases on a wide range of crop plants. Increasing genomic and transcriptomic resources and recent advances in oomycete biology demand new strategies for genetic modification of oomycetes. Most oomycete transformation procedures rely on geneticin-based selection of transgenic strains.
RESULTS
We established N-acetyltransferase AAC(3)-I as a gentamicin-based selectable marker for oomycete transformation without interference with existing geneticin resistance. Strains carrying gentamicin resistance are fully infectious in plants. We further demonstrate the usefulness of this new antibiotic selection to super-transform well-characterized, already fluorescently-labelled P. palmivora strains and provide a comprehensive protocol for maintenance and zoospore electro-transformation of Phytophthora strains to aid in plant-pathogen research.
CONCLUSIONS
N-acetyltransferase AAC(3)-I is functional in Phytophthora oomycetes. In addition, the substrate specificity of the AAC(3)-I enzyme allows for re-transformation of geneticin-resistant strains. Our findings and resources widen the possibilities to study oomycete cell biology and plant-oomycete interactions.
DOI: 10.1186/s12866-019-1642-0
PubMed: 31775609
PubMed Central: PMC6882347
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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scheme="MESH" xml:lang="fr"><term>Colorants fluorescents</term><term>Maladies des plantes</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Oomycetes are pathogens of mammals, fish, insects and plants, and the potato late blight agent Phytophthora infestans and the oil palm and cocoa infecting pathogen Phytophthora palmivora cause economically impacting diseases on a wide range of crop plants. Increasing genomic and transcriptomic resources and recent advances in oomycete biology demand new strategies for genetic modification of oomycetes. Most oomycete transformation procedures rely on geneticin-based selection of transgenic strains.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>We established N-acetyltransferase AAC(3)-I as a gentamicin-based selectable marker for oomycete transformation without interference with existing geneticin resistance. Strains carrying gentamicin resistance are fully infectious in plants. We further demonstrate the usefulness of this new antibiotic selection to super-transform well-characterized, already fluorescently-labelled P. palmivora strains and provide a comprehensive protocol for maintenance and zoospore electro-transformation of Phytophthora strains to aid in plant-pathogen research.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>N-acetyltransferase AAC(3)-I is functional in Phytophthora oomycetes. In addition, the substrate specificity of the AAC(3)-I enzyme allows for re-transformation of geneticin-resistant strains. Our findings and resources widen the possibilities to study oomycete cell biology and plant-oomycete interactions.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31775609</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>06</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2180</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>11</Month><Day>27</Day></PubDate></JournalIssue><Title>BMC microbiology</Title><ISOAbbreviation>BMC Microbiol</ISOAbbreviation></Journal><ArticleTitle>N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans.</ArticleTitle><Pagination><MedlinePgn>265</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12866-019-1642-0</ELocationID><Abstract><AbstractText Label="BACKGROUND">Oomycetes are pathogens of mammals, fish, insects and plants, and the potato late blight agent Phytophthora infestans and the oil palm and cocoa infecting pathogen Phytophthora palmivora cause economically impacting diseases on a wide range of crop plants. Increasing genomic and transcriptomic resources and recent advances in oomycete biology demand new strategies for genetic modification of oomycetes. Most oomycete transformation procedures rely on geneticin-based selection of transgenic strains.</AbstractText><AbstractText Label="RESULTS">We established N-acetyltransferase AAC(3)-I as a gentamicin-based selectable marker for oomycete transformation without interference with existing geneticin resistance. Strains carrying gentamicin resistance are fully infectious in plants. We further demonstrate the usefulness of this new antibiotic selection to super-transform well-characterized, already fluorescently-labelled P. palmivora strains and provide a comprehensive protocol for maintenance and zoospore electro-transformation of Phytophthora strains to aid in plant-pathogen research.</AbstractText><AbstractText Label="CONCLUSIONS">N-acetyltransferase AAC(3)-I is functional in Phytophthora oomycetes. In addition, the substrate specificity of the AAC(3)-I enzyme allows for re-transformation of geneticin-resistant strains. Our findings and resources widen the possibilities to study oomycete cell biology and plant-oomycete interactions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Evangelisti</LastName><ForeName>Edouard</ForeName><Initials>E</Initials><Identifier Source="ORCID">0000-0002-7218-7850</Identifier><AffiliationInfo><Affiliation>Sainsbury Laboratory Cambridge University (SLCU), Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yunusov</LastName><ForeName>Temur</ForeName><Initials>T</Initials><Identifier Source="ORCID">0000-0001-9936-4354</Identifier><AffiliationInfo><Affiliation>Sainsbury Laboratory Cambridge University (SLCU), Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shenhav</LastName><ForeName>Liron</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0003-1961-4187</Identifier><AffiliationInfo><Affiliation>Sainsbury Laboratory Cambridge University (SLCU), Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schornack</LastName><ForeName>Sebastian</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-7836-5881</Identifier><AffiliationInfo><Affiliation>Sainsbury Laboratory Cambridge University (SLCU), Cambridge, UK. sebastian.schornack@slcu.cam.ac.uk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Microbiol</MedlineTA><NlmUniqueID>100966981</NlmUniqueID><ISSNLinking>1471-2180</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005839">Gentamicins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>A08F5XTI6G</RegistryNumber><NameOfSubstance UI="C010680">antibiotic G 418</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.5</RegistryNumber><NameOfSubstance UI="D001191">Arylamine N-Acetyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.5</RegistryNumber><NameOfSubstance UI="C089384">N-acetyltransferase 1</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001191" MajorTopicYN="N">Arylamine N-Acetyltransferase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004351" MajorTopicYN="N">Drug Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005839" MajorTopicYN="N">Gentamicins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Aminoglycoside</Keyword><Keyword MajorTopicYN="Y">Counter-selection</Keyword><Keyword MajorTopicYN="Y">N-acetyltransferase</Keyword><Keyword MajorTopicYN="Y">Oomycete</Keyword><Keyword MajorTopicYN="Y">Specificity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>11</Month><Day>14</Day></PubMedPubDate><PubMedPubDate 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